EPSRC Case Studentship: Protein Engineering In Droplet Micro-Compartments, UK
Job Description: A graduate studentship leading to the award of a PhD is available to work at the Biochemistry Department at Cambridge University (with Dr F. Hollfelder) and at MedImmune’s Granta Park Site (with Drs D. Lowe, L. Jermutus and R. Minter.) The research project will involve work on protein engineering methods that take advantage of compartmentalisation in water-in-oil microdroplets. There is a strong interdisciplinary component, as devices for droplet handling will be self-made in the Cambridge Nanoscience Centre. The project will be supervised jointly by academic and industrial advisors and experimental work carried out both at the University and at MedImmune. Applicants should have a first or upper second class degree in chemistry, biochemistry or related subjects. Specific skills in protein engineering, cloning, DNA manipulation (including preparation and handling of DNA libraries) and familiarity with other molecular biology techniques would be advantageous.
The project is embedded in a range of European collaborations and will form part of the European Research Training Network on Directed Evolution of Functional Proteins (ENEFP). It is also part of a larger multi-disciplinary research initiative on microdroplets in microfluidic devices based in Cambridge. These initiatives provide special training programmes and exchange periods in other European laboratories (Strasbourg, Louvain-la-Neuve, Lausanne and Israel amongst others) and industrial partners. This studentship is funded by the EPSRC for up to 42 months. Recent Literature:
1. Schaerli, Y.; Hollfelder, F., The potential of microfluidic water-in-oil droplets in experimental biology. Mol Bio Sys 2009, 5 (12), 1392-404.
2. Stein, V.; Hollfelder, F., An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res 2009, 37 (18), e122.
3. V. Stein, I. Sielaff, K. Johnsson and F. Hollfelder (2007), A Covalent Chemical Genotype-Phenotype Linkage for in vitro Protein Evolution, ChemBioChem, 8(18), 2191-4.
4. A. Huebner, S. Sharma, M. Srisa-Art, F. Hollfelder, J. B. Edel, A. J. de Mello (2008) Microdroplets: A sea of applications? Lab Chip. 8(8):1244-54.
5. H. Leemhuis, V. Stein, A. D. Griffiths and F. Hollfelder (2005), New Genotype-phenotype Linkages for Directed Evolution of Functional Proteins, Curr Op. Struct. Biol., 15:472-478.
Applications should contain a CHRIS/6 form with the names and addresses of two referees and a CV, and should be sent quoting reference PH06799 by 13 July 2010 by email to fhsec@mole.bio.cam.ac.uk or by post to Mrs Anne Procter, Department of Biochemistry, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
* Limit of tenure: Up to 42 months.
Closing date: 13 July 2010.
Vacancy Reference No: PH06799
Further Scholarship Information
Scholarship Tags: Biochemistry, Engineering, Nanoscience, UK
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