This is Dr. Jayappa, completed doctoral degree in the year 2008. I worked on “Data base Development, Dataanalysis and explorig insect species for their antimicrobial activity”. Now i crawling for Postdoc positions in this University and would like to continue research programme in the same line. I would ready to develop research project on the same if any considered my request. So I request your kind self to guide and suggest me how to go about. I would greately appreciate you if you could make it.

Thanking you

Regards
Jayappa, J

Karnataka, India

Dear Sir,
Sub: Requisition of Post Doctoral position-reg.
I am Dr. V. Thiagarajan, writing to you to explore a possibility to get a Post doctoral position. I have completed my Ph. D in University of Madras (Microbiology-Botany ) at Centre for Advanced Studies in Botany, Chennai, India at December 2008. The thesis entitled “Production, purification and characterization of chitinase from Streptomyces sp. PTK19 and its biocontrol efficacy against Fusarium wilt of Lycopersicon esculentum”. I am herewith enclosing my detailed resume for your kind perusal and will be glad to submit additional information / document, if any required in support of my application. Given the opportunity, I am sure I will be able to contribute significantly. Waiting for your kind response.
CURRICULUM VITAE
Dr. V. Thiagarajan

# 10/626, Bharathi Street,
Mugappair East,
Chennai-600 037,
Tamil Nadu,
India.
E-mail: thiagusiva2002@yahoo.co.in ; Mobile:+91-9380731031

EDUCATIONAL QUALIFICATION

Examination passed Subject College / University Year of Passing Marks
(%)
Ph.D.,
Microbiology-Botany
interdisciplinary
Centre for Advanced Studies in Botany, University of Madras, India

December 2008 -
M.Sc., Microbiology JamalMohamed College, Trichy, Affiliated to Bharathidasan University, India April 2002 76.0
B.Sc., Microbiology Adhiparasakthi College of Science, Kalavai, Affiliated to University of Madras, India April 2000 78.6
H.Sc., Physics, Chemistry, Biology and Mathematics Tamil Nadu state Board, India March 1997 54.08
SSLC Mathematics, Science , Social science Tamil Nadu state Board, India
April 1994 83.2

Ph.D., Thesis:
Production, purification and characterization of chitinase from Streptomyces sp. PTK19 and its biocontrol efficacy against Fusarium wilt of Lycopersicon esculentum
Abstract of Ph.D., thesis:
Isolation and screening of chitinase producing Streptomyces sp. from soil and optimization of cultural conditions at different substrate concentrations, pH, temperature, nutrients namely carbon, nitrogen and amino acid supplementation under shaking condition. Attempts were also made for the production of chitinase in laboratory scale stirred bioreactor. The chitinase obtained from the liquid culture broth of Streptomyces sp. PTK19 was subjected for purification. The enzyme was purified through Ion exchange chromatography and gel filtration chromatography techniques. The molecular mass of purified chitinase was determined at 31.1 kDa using SDS-PAGE by comparing the mobilities of standard protein markers run concurrently. The properties namely pH, temperature optima, substrate specificity, stability and metal ions and chemical reagents inhibitors of purified chitinase were recorded. The Fusarium oxysporum PTK2 strain was used for the pot experiments. Dual plate method was conducted, the pot study experiments proved the biocontrol efficacy of Streptomyces sp. PTK19 against Fusarium wilt of Lycopersicon esculentum. The incidence of disease was measured on the basis of wilt symptoms. The Induced Systemic Resistance (ISR) components of the defense enzyme content were analyzed like peroxidase, polyphenoloxidase and chitinase from the leaf samples of Lycopersicon esculentum at 15, 20 and 25 days of old plants after transplantation and the experiments were conducted for a period of 90 days. The biocontrol efficacy of Streptomyces sp. PTK19 was proved.

Technical Experience
? Isolation, Identification and Maintenance of Microorganisms
? Production of industrially important enzyme from microorganisms
? Ion-exchange and Gel filtration chromatography
? SDS-PAGE and Native PAGE
? DNA and plasmid DNA isolation
? Serological Diagnosis, ELISA and Western blotting
? Biological control of Plant Pathogens viz., Fungi and Induced Systemic Resistance (ISR)
? Production of pigments from microorganisms
? Purification and characterization of microbial pigments

Working experience
Worked as Research Associate in Department of Biotechnology government of India funded project entitled “Hyper production of fungal pigments from lower fungi” during 5th June 2008 to 22nd September 2008 at Murugappa chettiar research center, Tharamani, Chennai, India.

Areas of interest
? Enzymology – Drug development using enzymes
? Immunology- Production of vaccines and vaccine development
? Virology -Anti HIV, Anti HBV drug development using chitinases
? Parasitology – Drug development
? Anti cancer drug development

ADDITIONAL QUALIFICATIONS
Diploma in Medical Laboratory Technology (DMLT) – Affiliated to AIMLTA Board New Delhi
Worked as a resource person for following workshops and training programmes conducted by University of Madras
S. No Title Date
1. Industrial products through fermentation Technology and Bio – Reactors Sep 26- 30, 2003
2 Industrial products through fermentation Technology and Bio – Reactors Oct 10-14, 2003
3. Industrial products through fermentation and Bio-reactors Dec 19-23, 2003
4. Wet lab training in Bio-informatics Dec 1 –12, 2003
5. Winter workshop on Microbial biotechnology and Bio-informatics Dec 22-31, 2003
6. Bioprocessing Technology (with Scigenics fermentor company) Feb 8-12, 2004
7. Bioprocessing Technology (with Scigenics Fermentor company) Mar 9-13,2004
8. Bioprocessing Technology (with Scigenics fermentor company) June 28 –July7
2004
9. Workshop on Bioprocessing Technology (Batch – IV, Batch -V) Dec 3 – 6, 2004
Dec 27 – 30, 2004
10 Plant Biology, Industrial Microbiology and Biotechnology Dec 1-Jan 31, 2004
11. Summer training in Biotechnology May –June, 2004
12 Advanced Bio-Technology May –June, 2004
13. Summer workshop on Microbial Biotechnology and Bio -informatics July 20-29, 2004
14. Fungal Biotechnology Batch I Aug 23-29, 2004
15. Fungal Biotechnology Batch -II Oct 20 –26, 2004
16. Molecular biology and its application in Biotools (SBRI) Sep 20-29, 2004
17. Monthly one day seminar on Medicinal plants cultivation and marketing Nov 14, Jan 7, 2004
18. Self supportive seminar cum workshop on recent trends in medicinal plants (Tamil) April 13-14, 2004
19. Training in plant Biology, Biotechnology and industrial Biotechnology (Batch I, Batch II, Batch III) Oct – Dec, 2004
Jan – Mar, 2005
20. Second winter workshop on Microbial Biotechnology and Bioinformatics (Batch I, Batch II) Dec 17 – 20, 2004
Jan 7 –10, 2005
21. Workshop on Application of Bioinstruments Dec 21-23, 2004
Jan 27 – 29, 2005
Feb26-28, 2005
22. Workshop on Microbial Biotechnology and Bioinformatics 14 – 18 July, 2005
23. Workshop On Microbial Biotechnology (Batch I) 19 to 23 June 2006
24. Workshop On Microbial Biotechnology (Batch II) 24-28, July, 2006
25. Workshop On Modern Trends in Molecular Biology, Sponsored by Science City, Chennai. 9 – 31 May, 2007
26. Workshop On Microbial Biotechnology (Batch I) 6-11, July, 2007
27. Workshop On Microbial Biotechnology (Batch II) 3-8, Sep, 2007

WORKSHOPS/CONFERENCE
1. Attended and presented a poster paper presentation and won the first prize on 3rd National Conference of Indian Association of Applied Microbiologists (IAAM), conducted by Adhiparasakthi College of Arts and Science, Vellore.
2. Attended a national Conference on ‘Recent Trends on Medicinal Plants Research’ conducted by CAS in Botany, Guindy campus, Chennai.
3. Attended Workshop on ‘Microbial Biotechnology and Bioinformatics’ conducted by CAS in Botany, Guindy campus, Chennai.
4. Attended training programme in ‘Molecular Biological Tools and Bioinformatics’ conducted by CAS in Botany, Guindy campus, Chennai.

PERSONAL INFORMATIONS
Father’s Name : M.Venkatesan
Sex & Marital status : Male & Unmarried
Date of Birth & Age : 05.07.1979 & 29
Nationality & Religion : Indian & Hindu
Passport Number : G1049974
Languages known : Tamil, English& Telugu

REFERENCES
Prof. P.T. Kalaichelvan, Supervisor
Centre for Advanced Studies in Botany
University of Madras, Guindy Campus
Chennai – 600 025, India.
Mobile: +91-9381033198
Email: ptkalai2003@yahoo.com
Prof. R. Rengasamy, Director
Centre for Advanced Studies in Botany
University of Madras, Guindy Campus
Chennai – 600 025, India.
Email: profrrengasamy@yahoo.com
Mobile :+91-9283445271
Declaration
Hereby, I declare that the above given details are true to the best of my knowledge.
Yours Sincerely,
(V.THIAGARAJAN)

Expecting your prompt response at the earliest.

Thanking You

With Warm Regards

Sincerely Yours
Dr.Bibhu Prasad Rautray

hailishye@yahoo.ca